A simple, precise and reproducible method has been developed and validated for the estimation of Ondansetron hydrochloride in pharmaceutical dosage forms by RP-HPLC. The separation was achieved using INERTSIL C8 (250mm x 4.6mm, 5�µ particle size) in isocratic mode with mobile phase acetonitrile: water (60:40 v/v); pH was adjusted to 2.5 with 0.1% orthophosphoric acid at a flow rate of 1.0ml/min. Detection was carried out at 247nm using PDA detector. The retention time was found to be 3.56min. The system suitability parameters such as theoretical plate count, tailing and percentage RSD between six standard injections were within the limit. The method was validated according to ICH guidelines. The method was linear in the concentration range of 50-150 �µg/ml with a correlation coefficient of 0.999. The percentage recovery study of ondansetron hydrochloride was found to be 99.59-101.34% respectively. The proposed method can be used for routine analysis
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